Development of low-cost rapid pathogen detection microsystem in COVID-19 Pandemic: Micro PCR module- Bioengineering Approach
Project Description

Due to the massive outbreak of Coronavirus-19 worldwide, rapid and accurate detection of pathogenic microorganisms has become a top priority. Compared to the current centralized detection mode, miniaturized detection platforms could enable real-time and on-spot testing of diseases, which could reduce the unnecessary pressure on medical system and avoid the possible contamination of samples during shipment. Our research team wants to integrate the CMOS ion-sensitive field-effect transistors (ISFETs) with microfluidics to propose a rapid electrochemical detection system for COVID-19. The microfluidics will take part of DNA extraction from samples and the on-chip PCR while the ISFETs will be used to detect the pH change during the amplification process. Subsequently, the system can also be used to establish an IoT platform for more convenient and effective data management by integrating Bluetooth modules with the cloud for wireless data transfer.

Supervisor
LEE Yi-Kuen
Quota
4
Course type
UROP1000
UROP1100
UROP2100
UROP3100
UROP4100
Applicant's Roles

Successful candidates are expected to understand the basic principle of the design of micro PCR chip and the temperature control system and to conduct the experiments for comparative testing of on-chip PCR microfluidic module and commercial real-time PCR system.
Tasks to be done in this project:
• Understand the basic principle of micro PCR and temperature control system design
• Work with the others for the integration of micro PCR with micro DNA extraction and ISFET sensor design
• Conduct comparison testing of micro PCR and the commercial real-time PCR system

Applicant's Learning Objectives

• Improve skills in laboratory including real-time PCR and micro PCR module
• Get knowledge in integration of micro PCR with micro DNA extraction and ISFET sensor
• Acquire hands-on experimental skills in testing of micro PCR and real-time PCR system

Complexity of the project
Moderate