Due to the massive outbreak of Coronavirus-19 worldwide, rapid and accurate detection of pathogenic microorganisms has become a top priority. Compared to the current centralized detection mode, miniaturized detection platforms could enable real-time and on-spot testing of diseases, which could reduce the unnecessary pressure on medical system and avoid the possible contamination of samples during shipment. Our research team wants to integrate the CMOS ion-sensitive field-effect transistors (ISFETs) with microfluidics to propose a rapid electrochemical detection system for COVID-19. The microfluidics will take part of DNA extraction from samples and the on-chip PCR while the ISFETs will be used to detect the pH change during the amplification process. Subsequently, the system can also be used to establish an IoT platform for more convenient and effective data management by integrating Bluetooth modules with the cloud for wireless data transfer.
Successful candidates are expected to understand the basic principle of the design of micro PCR chip and the temperature control system and to conduct the experiments for comparative testing of on-chip PCR microfluidic module and commercial real-time PCR system.
Tasks to be done in this project:
Understand the basic principle of micro PCR and temperature control system design
Work with the others for the integration of micro PCR with micro DNA extraction and ISFET sensor design
Conduct comparison testing of micro PCR and the commercial real-time PCR system
Improve skills in laboratory including real-time PCR and micro PCR module
Get knowledge in integration of micro PCR with micro DNA extraction and ISFET sensor
Acquire hands-on experimental skills in testing of micro PCR and real-time PCR system