Development of low-cost rapid pathogen detection microsystem in COVID-19 Pandemic: Micro/nano DNA extraction module- Microfluidics Approach
Project Description

Due to the massive outbreak of Coronavirus-19 worldwide, rapid and accurate detection of pathogenic microorganisms has become a top priority. Compared to the current centralized detection mode, miniaturized detection platforms could enable real-time and on-spot testing of diseases, which could reduce the unnecessary pressure on medical system and avoid the possible contamination of samples during shipment. Our research team wants to integrate the CMOS ion-sensitive field-effect transistors (ISFETs) with microfluidics to propose a rapid electrochemical detection system for COVID-19. The microfluidics will take part of DNA extraction from samples and the on-chip PCR while the ISFETs will be used to detect the pH change during the amplification process. Subsequently, the system can also be used to establish an IoT platform for more convenient and effective data management by integrating Bluetooth modules with the cloud for wireless data transfer.

Supervisor
LEE Yi-Kuen
Quota
4
Course type
UROP1000
UROP1100
UROP2100
UROP3100
UROP4100
Applicant's Roles

Successful candidates are expected to work on micro/nano DNA extraction chip design, including review of various technologies for on-chip DNA extraction which can be used for the integration with on-chip micro PCR and integrated ISFET sensor, perform quantitative design analysis using commercial software: Solidworks and/or COMSOL.
Tasks to be done in this project:
• Review of different technologies for on-chip DNA extraction
• Come up with a preliminary design
• Simulation and testing of the prototype
• Integration with micro PCR module

Applicant's Learning Objectives

• Improve skills in mechanical design using SolidWorks (widely used in industry)
• Learn how to do simulation with COMSOL
• Acquire experience on design and testing of microfluidic

Complexity of the project
Moderate