The superfamily of G protein-coupled receptors (GPCRs) constitute the largest cell-surface detectors for hormones, neurotransmitters, photons, and numerous other extracellular signals. In order to thoroughly understand how the GPCRs regulate cellular functions, specific combinations of receptors and G protein subunits will be expressed in mammalian cell lines, and their effects on downstream effectors assessed by measuring the activities of selected enzymes, kinases, and ion channels.
The applicant is expected to assist in generating research tools (e.g., GPCR and G protein mutants) and perform routine experiments. The applicant will work with an experienced researcher. A good understanding in cell biology is preferred.
The learning objectives for the project include, but not limited to, the following: 1. Standard laboratory skills (e.g., preparing reagents) 2. Basic recombinant DNA techniques (e.g., restriction digests) 3. Basic protein chemistry techniques (e.g., SDS-PAGE) 4. Planning and execution of experiments 5. Data analysis and presentation 6. Foundation in cell signaling