Influence of random base in poly(A) tail to the function of mammalian mRNA
Project Description

Recent research discovers that some mRNAs in human cells has poly(A) tails that are not 100% made of Adenine. Single insertion of G, U, or C has been found. Sequencing techniques revealed that mRNAs has such random insertion tend to have a longer half-life in cell. But is this elongated half-life really stem from such insertion? And how do such insertions affect the function of mRNAs?

Supervisor
KUANG Becki Yi
Quota
2
Course type
UROP1000
Applicant's Roles

RNA design and synthesis; mammalian cell culture; RNA transfection and expression measurement; data processing.

Applicant's Learning Objectives

To learn how to successfully design an artificial RNA with expect functions in cell; to understand the role of each structural elements of an mRNA.

Complexity of the project
Challenging