Recent research discovers that some mRNAs in human cells has poly(A) tails that are not 100% made of Adenine. Single insertion of G, U, or C has been found. Sequencing techniques revealed that mRNAs has such random insertion tend to have a longer half-life in cell. But is this elongated half-life really stem from such insertion? And how do such insertions affect the function of mRNAs?
RNA design and synthesis; mammalian cell culture; RNA transfection and expression measurement; data processing.
To learn how to successfully design an artificial RNA with expect functions in cell; to understand the role of each structural elements of an mRNA.