Transcription is the first step and the pivotal step in the regulation of gene expression. The goal of this project is to study how RNA polymerase transcribes through the DNA using single-molecule optical tweezers and how transcription factors are involved in this process.
Prepare the DNA template and proteins for the single-molecule transcription elongation assay. Students will work with other lab members in a collaborative way.
Students will learn to how to approach research problems with an analytical approach and develop scientific thinking skills. Students will receive training in modern molecular biological and biophysical methods through the project.