Transient receptor potential channels (TRPCs) are a group of non-selective ion channels, which play important roles in various cellular activities. Our project is aiming to study the role of TRPCs in the neurogenesis of mouse embryonic stem cells (mESCs). Genetic manipulation will be applied firstly to knock down or overexpress TRPCs with CRISPR/Cas9. Then several neural differentiation markers will work as the readout of neurogenesis from mESCs to explore the effect of genetic manipulation on TRPCs.
The applicant must have curiosity to scientific research as well as good time management ability to arrange the lab work. The applicant is supposed to design the oligos for CRISPR/Cas9 system and make successful cloning vectors then drive the expression of TRPCs knockdown or overexpressing in mouse ES cells. Following Western blotting or qRT-PCR will be applied to detect the efficiency of above genetic manipulation. After that, the effects of TRPCs on neurogenesis will be detected with Western blotting, qRT-PCR and immunofluorescence staining.
1. How to do literature review in a specialized research area. 2. Know the basic laboratory rules and regulations. 3. Hand-on experience of molecular and cellular biology.