The non-random distribution of 5-hydroxymethyluracil in dinoflagellates
Project Description

The non-random distribution of 5-hydroxymethyluracil in dinoflagellates
The dinoflagellates not only have genome sizes up to 80-100 times that of human beings (up to 250 vs 3 pg of haploid genome size).
• The haploid genome of dinoflagellates contained 70% modified and rare bases non-randomly distributed in the genome (Díaz Espina et al., 2005)
• The distribution of 5-hmU is not uniform (Rae, 1973) showed by buoyant density fractions (Steele & Rae, 1980)
• Dinoflagellates possess special cation-superhelicity-mediated DNA condensation in their non-nucleosomal anisotropic chromosomes
• 5-hmU is being found only in DNA of dinoflagellates so far and its level vary among species (Steele & Rae, 1980)
• 5-hmU level in spermatocytes, which also have highly anisotropic organization, is more than ten-fold higher than in somatic cells(Olinski, Starczak & Gackowski, 2016)
• The presence of 5-hmU does not apparently interfere with polymerasefunction (Olinski, Starczak & Gackowski, 2016)

Fojtová, M., Wong J.T.Y., Dvo?á?ková, M., Yan, K.T.H., Sýkorová, E., and FajkusJ. (2010). Telomere maintenance in liquid crystalline chromosomes of dinoflagellates. Chromosoma.
Mak, C.K.M., Hung, V.K.L., and Wong, J.T.Y. (2005). Type II topoisomerase activities in both the G1 and G2/M phases of the dinoflagellate cell cycle. Chromosoma 114, 420-431.
Wong, J.T.Y., and Kwok, A.C.M. (2005). Proliferation of dinoflagellates: blooming or bleaching. Bioessays 27, 730-740.
Yeung, P.K.K., New, D.C., Leveson, A., Yam, C.H., Poon, R.Y.C., and Wong, J.T.Y. (2000). The Spindle Checkpoint in the Dinoflagellate Crypthecodinium cohnii. Exp Cell Res 254, 120-129.
Moreau H., Géraud M.L., Bhaud Y., Soyer-Gobillard M.O. (1998) Cloning, characterization and chromosomal localization of a repeated sequence in a dinoflagellate : Crypthecodinium cohnii Biecheler. Internatl. Microbiol.1:35-43.
Dinoflagellates are amazing group of organisms for many other reasons: Dinoflagellates are also the primary producer of the coral reef community (as symbiotic zooxanthallae) and major causative agents of red tides and many natural products (e.g. Omega-3, Dinoflagellates are also the sister group to many major human parasites, including Plasmodium ( malaria) and Toxoplasma.(Toxoplasmosis).

Supervisor
WONG Joseph Tin Yum
Quota
1
Course type
UROP1000
Applicant's Roles

The project will involve cell biological and molecular techniques in the generation and monitoring of dinoflagellate genomic DNAs

To conduct literature review and experiments under directions
under the auspices of the learning objectives

Applicant's Learning Objectives

To interact with other laboratory members in a working environment.
To operate time management as to the conduct of the laboratory
Laboratory safety and disposal management

Literature review in a frontier subject area
Appreciate the interpretation of experimental results and the translation to scientific theory

Complexity of the project
Challenging