The dynamic balance between excitation and inhibition in synaptic transmission is necessary for proper functions of central nervous system (CNS). Inhibitory synaptic transmission, which is mediated mainly by GABA and glycine, regulates the neuronal excitability. Inhibitory synaptic transmission is reported to considerably different from excitatory synaptic transmission. However, the inhibitory synaptic transmission compared to the excitatory synaptic transmission remains poorly understood. In particular, the precise and dynamic motion of inhibitory neurotransmitter receptors during remains unknown. In this study, we will investigate the dynamics of GABAergic receptors in mature rat hippocampal neurons by real-time tracking of single GABAergic receptors before, during and after synaptic transmission
Understand how to track single GABAergic receptors in living neurons.