Small, ubiquitous, and beautiful
Project Description

Small, Ubiquitous, and beautiful
Membranes are the margin of life, defining the size and potential of the cells.
Membrane proteins are commonly large, doing chores like ion transporting, receptoring hormones or sensing the five senses. With one exception.
The Plasma Membrane Proteolipid 3 (PMP3, UPF0057 family in Uniprot) family consists
of abundant small hydrophobic polypeptides with only two predicted transmembrane helices. https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920…

We recently reported abundant expression of Group I PMP3 homologues (PMP3(i)hs) during normal vegetative growth in both prokaryotic and eukaryotic cells, at a level comparable to housekeeping genes, implicating the regular cellular functions. Expression of eukaryotic PMP3(i)hs was dramatically upregulated in response to membrane potential (Vm) variability (Vmvar),whereas PMP3(i)hs deletion-knockdown led to Vm changes with conditional growth defects.
These data demonstrated a direct reciprocal relationship between PMP3(i)hs expression and Vm differentials in both prokaryotic and eukaryotic cells.

The project will have a bioinformatic component investigating potential homologues, and depending on the student and COVID-19, progressing to a laboratory component in transgenic expression.

Supervisor
WONG Joseph Tin Yum
Quota
2
Course type
UROP1000
UROP1100
Applicant's Roles

The project will have a bioinformatic component investigating potential homologues, and depending on the student and COVID-19, progressing to a laboratory component in transgenic expression.

Applicant's Learning Objectives

bioinformatic analysis, organizing internet public domain data
critical analysis of taxonomic group differences and predicted functions
interested students can progress to protein expression experiments.

Complexity of the project
Challenging