The ATM and ATR proteins play unexpected roles in the movement of vesicles in mammalian cells. Both proteins have homologs in yeast and this project will explore the extent to which the power of yeast genetics can be used to predict the behavior of vesicles in mammalian cells, including synaptic vesicles in neuronal synapses.
UROP1100 UROP2100 UROP3100 UROP4100
The applicant will learn how to culture yeast and image the process of vesicle endocytosis using dye uptake techniques. The parameters of this process will the be tested for their response to genetic, epigenetic and metabolic factors
Applicant's Learning Objectives:
Learn the techniques of yeast culture
Learn to manipulate the yeast genome
Learn the fundamentals of vesicle trafficking in all cells
Explore the parallels in vesicle trafficking regulation in mammalian and yeast cells