An exploration of nuclear dynamics in cells and syncytia of developing zebrafish embryos
MILLER Andrew L
HO Sarah E
The nuclei of cells often associated with a peri-nuclear ER, which in some cases has been reported to be continuous with the nuclear membrane. In addition to its other functions, the ER serves a one of the major storage sites for intracellular Ca2+. Nuclei are also dynamic organelles from a number of perspectives, thus moving them and their associated Ca2+ store around cells might potentialy act as a means of localizing and directing Ca2+ signals. This may be especially so in multinucleate cells and syncytia. In this project you will explore possible mechanisms for moving nuclei around in cells and snyncytia as well as to investigate whether shuch movements may act as a mechanism to proagate Ca2+ waves in cells and syncytia. This will involve applying a variety of microscopy techniques to visualize both nuclear movement and propagating Ca2+ waves.
Applicant will be instructed in basic zebrafish husbandry as well as crossing adult fish to generate fertilized eggs. They will also be instructed in basic fluorescent microscopy as well as video microscopy. Excellent hands and good observational skills are required, but instruction will be provided to develop these skills.
Applicant's Learning Objectives:
The major learning objective is to understand an apply scientific principles to problem solving: Developing a hypothesis, then testing its validity. Understanding the value of planning; good experimental techniques; the importance of controls; recording data; and subsequent analysis will also be important learning objectives.