Alternative models have been proposed regarding the mechanism that controls the numbers of individual types of neurons in the brain. This project examines the lineage model which posits that factors intrinsic to the neurons are the sole determinants of the cell number. The experimental techniques involved in this project include tissue homogenization, immunostaining and fluorescence microscopy. Participants who have the interest and basic skills in animal dissection may perform mouse brain dissection as well. Some participants are expected to perform statistical analysis on the experimental data to evaluate if they fit the lineage model.
UROP1100 UROP2100 UROP3100 UROP4100
All participants of the project are expected to acquire basic understanding of the scientific background of this project through reading scientific articles and/or book chapters assigned by the supervisor and attending tutorial, discussion and demonstration sessions conducted by the supervisor and research associates. All participants are also expected to participate in regular group meetings to share experience and ideas, and to write a report on the project according to the guidelines stipulated by the supervisor. While some participants will focus on the laboratory work to generate experimental data and others on the development of a statistical model for analyzing the experimental data, all participants will be involved in interpreting the data.
Applicant's Learning Objectives:
On completion of this project, participants are expected to be able to
• outline the embryonic development of central nervous system in mammals,
• identify main parts of mammalian brain and main types of cells in it,
• demonstrate competence in laboratory techniques and/or statistical analysis involved in this project,
• communicate effectively and collaborate productively with peers and seniors in teamwork,
• generate, describe, analyze, interpret and explain experimental data based on scientific reasoning and knowledge, and in compliance with ethical principles.